What is an ELISA Test Kit? | |
An ELISA test kit consists of all the necessary components to perform an ELISA assay, including microplate wells, assay buffers, standards, controls, and often a detailed protocol. The core principle of the ELISA technique is based on the specific binding between an antigen and an antibody. By leveraging enzyme-linked antibodies, ELISA tests can quantify the presence of specific biomarkers in a sample. ### Types of ELISA There are several variations of the ELISA technique, each tailored to specific applications: 1. **Direct ELISA**: This method involves immobilizing an antigen on a microplate and then adding an enzyme-linked antibody directly to quantify the antigen. 2. **Indirect ELISA**: In this method, the antigen is coated on the plate, and a primary antibody is added first followed by an enzyme-linked secondary antibody that binds to the primary antibody, providing enhanced sensitivity. 3. **Sandwich ELISA**: This is one of the most commonly used formats, where an antibody is coated on the plate, followed by the sample (which contains the target antigen) and a second enzyme-linked antibody, allowing for specific detection of the antigen. 4. **Competitive ELISA**: This type measures the concentration of an antigen by observing the competition between the sample antigen and a fixed amount of enzyme-linked antigen for a specific antibody. https://mzfoodtest.com/ ![]() | |
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